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KMID : 0895820110210010079
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Journal of Oriental Rehabilitation Medicine
2011 Volume.21 No. 1 p.79 ~ p.95
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Inhibiting Effects of Hwallak-dan(Huoluo-dan ) on the Adjuvant-induced Arthritis in Lewis Rats
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Kim Deok-Hee
Jeong Su-Hyeon
Seo Il-Bok
Kim Soon-Joong
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Abstract
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Objectives: This study was to investigate the antiarthritic effect of Hwallak-dan(Huoluo-dan) on the complete freund`s adjuvant(CFA)-induced arthritis in Lewis rats.
Methods: Arthritis was induced by intradermal injection of CFA into base of tail. Experimental groups divided into normal(n=10), control(n=10) and treated(n=10) group. Animals of control group received normal saline for twenty days, treated group received extracts Hwallak-dan(Huoluo-dan) for same duration, light mineral oil only injected non-arthritic rats were served as normal group. The incidence of arthritis and arthritic index were observed after treatment. Body weight, paw edema volume and thickness of ankle joint were measured at 0, 11, 14, 17, 20 days after treatment. White blood cell(WBC) counts in blood were analysed at 20 days after treatment and tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), IL-6 contents in paw exudate were analysed by ELISA at 20 days after treatment. Histopathology on the ankle joint were performed at 20 days after treatment.
Results: 1. Incidence of arthritis of treated group was 60% and control group was 100% at 20 days after treatment. 2. Arthritic index of treated group was significantly decreased compared with control group at 20 days after treatment. 3. Paw edema volume and thickness of ankle joint of treated group was significantly decreased compared with control group at 20 days after treatment. 4. Total WBC of treated group was significantly decreased compared with control group at 20 days after treatment. 5. Neutrophils of treated group was significantly decreased compared with control group at 20 days after treatment. 6. TNF-α content in paw exudate of treated group was significantly decreased compared with control group at 20 days after treatment. 7. IL-1β content content in paw exudate of treated group was significantly decreased compared with control group at 20 days after treatment. 8. IL-6 content content in paw exudate of treated group was significantly decreased compared with control group at 20 days after treatment. 9. Histopathological arthritic index of treated group was significantly decreased compared with control group at 20 days after treatment.
Conclusions: These results indicate that Hwallak-dan(Huoluo-dan) has inhibitory effect on the development and progression of CFA-induced arthritis in rats.
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KEYWORD
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Hwallak-dan(Huoluo-dan), Adjuvant arthritis, WBC, TNF-α, IL-1β, IL-6
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